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STAR-HTSeq RNA-seq processing pipeline environment and wrapper script, including SRA query, download, and caching functionality and useful reuse/restart features - hermidalc/rna-seq-star-htseq Contribute to Kingsford-Group/lrassemblyanalysis development by creating an account on GitHub. Contribute to mlbendall/TelescopeEncode development by creating an account on GitHub. XML metadata download and flat file conversion 1. Another ad-‐hoc script (get_SRA_run_and_sample_xml.pl) was run to download al of the SRP002395 study's run and sample metadata in XML format from the SRA web site and parse out the crucial… The list of run accessions can be entered in the SeqSphere+ Tools | Download Fastq from SRA dialog to download the metadata and the Fastq files.
The irony here is the this is possible to do for Gtex because the BigWig files are available for all samples from GTex on recount2. CCLE is not in recount2 yet. MISO subsequently quantifies the relative expression levels of alternatively polyadenylated isoforms and returns “ψ values,” which represent the expression levels of alternatively polyadenylated isoforms relative to that of all transcripts… Background Coral reefs are the most biodiverse ecosystems in the marine realm, and they not only contribute a plethora of ecosystem services to other marine organisms, but they also are beneficial to humankind via, for instance, their role… So start (in linux) by making a text file containing all the SRA file links fron the NCBI ftp site. Let's call it "url.txt". http://ftp-trace.ncbi.nlm.nih.gov/sra/sra-instant/reads/ByStudy/sra/SRP/SRP038/SRP038101/SRR1171523/SRR1171523.sra… We report that a subset of avian genes is characterized by very high GC content and long G/C stretches. These sequence characteristics correlate with the frequent absence of these genes from genomic databases. Exploring some scRNA-seq methodologies, not a full-fledged pipeline ready for use - AlexsLemonade/scRNA-seq_sandbox
We report that a subset of avian genes is characterized by very high GC content and long G/C stretches. These sequence characteristics correlate with the frequent absence of these genes from genomic databases. Exploring some scRNA-seq methodologies, not a full-fledged pipeline ready for use - AlexsLemonade/scRNA-seq_sandbox Alignment and filtering effects on RNAseq analysis on the X and Y chromosomes - WilsonSayresLab/XY_RNAseq ConsensusCruncher is a tool that suppresses errors in next-generation sequencing data by using unique molecular identifiers (UMIs) to amalgamate reads derived from the same DNA template into a consensus sequence. - pughlab/ConsensusCruncher All images show single optical sections and anterior is to the left. Dashed boxes highlight inset regions, magnified below. S7 and supplemental Table S5). Nearly 3000 gene products (1500 gene product groups) appear in all mixtures and represent highly homologous abundant proteins are fully shared cross-species.
#Read SRA file infos sri<-read.csv("SraRunInfo.csv", stringsAsFactors=False) files<-basename(sri$download_path) for(i in 1:length(files)) download.file(sri$download_path[i], files[i]) To download the raw read sequence data, note the SRA number on GEO: SRP090110 with study SRP002395) in this directory were generated through the fol owing process: 4.1.1. Download all 7518 runs in SRA native format from NCBI using Aspera client. 4.1.2. Convert all 7518 runs from SRA to SFF format using the "sffdump… A utility for easy downloading of reads from next-gen sequencing repositories like NCBI SRA - louiejtaylor/grabseqs Package for fetching metadata and downloading data from SRA - saketkc/pysradb You will be presented with a page for the overall SRA accession SRP064605 - this is a collection of all the experimental data.
Alignment and filtering effects on RNAseq analysis on the X and Y chromosomes - WilsonSayresLab/XY_RNAseq